Plates were coated with 5 μg/mL of MAb5.6 (100 μL/well), blocked, washed, and dispensed with 5 μL of the refolded proteins diluted in PBS-T with or without clomazone (100 ng/mL), after incubation (1 h), the wells were washed and dispensed with an anti-HA antibody coupled to peroxidase (HRP) (Roche, Madison, WI) or alternatively with biotin-HRP (Pierce).
Being buried in a cheap pine coffin and dispensing with a marquee at the funeral in order to cut costs are simply not an option.
Other strengths of the IADPSG criteria include a reduction in the patient burden by using a test of only 2-h duration and dispensing with a preliminary glucose challenge test a test chosen more for its convenience in a crowded clinic rather than for its relation to patient care.
Looking down, offering monosyllabic answers, and dispensing with an opening statement, the general left little doubt that he was simply carrying out orders.
Everyone seems to have forgotten that this woman is dangerous – not someone to be despised and dispensed with, but a sorceress with uncanny and terrible powers.
Opening tonight at Mike Weiss Gallery in Manhattan, Gray's Gymansty is an art show that preaches "self-help advice and encouragement dispensed with a heavy dose of nihilistic pragmatism" through performance, video, and sculpture.
So as Mr. Gephardt finished his orange roughy and the president dispensed with a hamburger and fries, Laura Bush, the first lady, appeared with a comely chocolate confection, lit by candles.
From this reference, we can deduce that the OLC authored a separate drone memo assessing – and dispensing with – the proposition that an American citizen had the right not to be deprived of his life without some form of judicial process.
The company is initially aiming for the owner-operators market — truck drivers who own their own rigs and would be able to increase their productivity by sleeping during long-haul trips and dispensing with the need for a second driver.
The days and nights were so warm he dispensed with a face mask or sleeping bag, curling up in the straw with the dogs.
