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For Salmonella sp., the crater-like data ring for food/water ontological terms was even more pronounced and differentiated with a number of higher detail data peaks, which were available for food aspects (pork, fresh poultry, fresh eggs, and vegetables) as well.
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HT-29 cells were maintained under standard culture conditions and differentiated with sodium butyrate.
THP-1 monocytes were seeded in T25 flask at 2.5 × 10 cells/T25 and differentiated with PMA.
The number of neuronal sphere-like colonies and differentiated colonies with a diameter greater than 50 um was counted by two independent scorers.
Briefly sections were treated with alum hematoxylin for 1 min, 0.5% phloxine in 0.5% aqueous calcium chloride for 20 min, and then finally differentiated with a saturated solution of tartrazine in 2-ethoxy ethanol (Sigma, St . Louis MO, USA).
This suggests that Sulcia is more terminally differentiated, with a static backbone gene order and slow gene content dynamics.
The houses facing the side street were picturesquely differentiated, with a conical roof over an open porch in the corner building, and then steep pitched roofs stretching down the row, punctuated by stepped gables.
Such selection could be socially differentiated, with a higher probability of abortion among advantaged women (27).
These groups were strongly differentiated, with a microsatellite based ϕst of 0.23 (p < 0.01) (Table 1).
Populations were highly differentiated, with a global FST value of 0.219.
Histological examination classified this tumour as well differentiated with a T3NxM1 stage.
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