The phrase "and differentiated with" is correct and usable in written English.
It can be used when describing how something is distinguished or set apart from something else, often in a technical or academic context.
Example: "The samples were treated with various chemicals and differentiated with specific markers to identify the different cell types."
Alternatives: "and distinguished by" or "and characterized by".
HT-29 cells were maintained under standard culture conditions and differentiated with sodium butyrate.
As she puts it in a famous passage from the Introduction to The Second Sex: "She is defined and differentiated with reference to man and not he with reference to her; she is the incidental, the inessential as opposed to the essential.
For these assays, we selected primary macrophages from human PBMC and THP-1 cell line, which has alveolar macrophage characteristics when stimulated and differentiated with PMA [30].
For myotube formation, C2C12 cells were cultured on gelatin-coated plates until confluence, and then washed with serum-free DMEM and differentiated with DMEM supplemented with 2% horse serum (HS, Invitrogen) and insulin (Sigma) for 4 6 days.
One day before the experiments, U937 cells were washed and resuspended in RPMI-1640 with 10% FCS (∼300,000 cells/ml) and differentiated with PMA (50 nM) as reported [32] [34].
THP-1 monocytes were seeded in T25 flask at 2.5 × 10 cells/T25 and differentiated with PMA.
Previous preliminary experiments confirmed that on days 14 and 21 differentiated with cortisone and days 8 and 16 differentiated with cortisol, cells had reached comparable differentiation states (assessed lipid accumulation and ΔCT values of differentiation markers) due to the greater differentiation capacity of the active steroid.
Thermally driven convection of solid satellite material as potential cause of surface evolution is considered for two cases: non-differentiated icy-mineral satellites and differentiated satellites with icy mantle and rocky core.
To see whether p53 from stem cells is acetylated, we treated stem cells and differentiated cells with the HDAC inhibitors trichostatin A and nicotinamide prior to cell lysis.
Treatment of stem cells and differentiated cells with trichostatin A/nicotinamide resulted in strong acetylation of p53 and an increase in overall p53 abundance (Supplementary Figure S4B).
We found that there were no differences in marker expression in spherical and differentiated cells, with the exception of two adhesion molecules, CD44 and CD54.
