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However, PDRN is liquid phase, and this study needed a scaffold where cells could be attached, grow, and differentiate in the cell differentiation of PDRN to investigate bone-forming capacity, a hard tissue, while previous studies focused on wound healing and regeneration of soft tissue.
Hydrogel scaffolds are highly hydrated polymer networks that allow cells to adhere, proliferate and differentiate in the treatment of diseased or injured tissues and organs.
The US retail market for apparel is ~$300 billion – there's good reason to believe that people's strong drive to personalize and differentiate in the real world will proliferate online as well.
The new generation of biomaterial scaffolds is being designed to recapitulate the local cytokine and/or growth factor milieu of development and remodeling, and to stimulate specific cell subsets to proliferate and differentiate in the desired and patient-specific way.
The biocompatibility of the scaffolds in vitro was studied, and it was found that the scaffolds showed no toxicity and that the cells could effectively attach, proliferate, and differentiate in the 3D skeleton of the scaffolds.
For subsequent experiments, THP-1 cell suspensions were adjusted to a concentration of 5×105 cells/ml in warm RPMI supplemented, seeded in 24-well plates adding 1 ml of suspension per well, and cells were allowed to adhere and differentiate in the presence of 10 ng/ml PMA (Sigma), at 37°C in 5% CO2 for 48 hours.
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When A2B5+ NPCs were treated with siRNA of both TRPC5 and TRPC6, the cells hardly proliferated and differentiated in the differentiation condition (Figure 6B).
In this study, the cells hardly proliferated and differentiated in the differentiation condition by simultaneous knock-down of TRPC5 and TRPC6, but the extent of inhibition against TG-stimulated Ba2+ influx was slightly decreased compared with the single knock-down of TRPC5.
Undifferentiated hESCs proliferated and differentiated in the PGSA scaffold.
Interestingly, although there is a good overall correlation between the data sets (Figure 3A) there are many mRNAs that exhibit differential decay rates between C2C12 cells and ES cells under the three conditions tested: pluripotent (+Leukemia Inhibitory Factor, LIF), differentiating in the absence of LIF, and differentiating in the presence of retinoic acid (RA) (Figure 3B).
Also, tissues are rapidly dividing and differentiating in the fetus, and such a high level of cell activity is vulnerable to disruption of normal development.
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