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Subgroup analyses were done by risk of bias, study duration, age group, UTI symptoms, world region and economy of country (categorised as developed and developing using the World Bank classification [ 26]).
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The CDS tool was designed and developed using the agile development methodology with the close engagement of the ASHAs and physicians.
The DNA was cross-linked to the membrane using a Stratagene cross-linker and developed using the chemiluminescent nucleic acid detection kit (Pierce) according to manufacturers instructions.
This new neural network structure is based on Spike Neural Networks and developed using the quaternion algebra.
The model is designed and developed, using the data, both from literature and our experiments on Kazakhstani fly ash.
The game was also moved fully onto eighth generation consoles and developed using the company's new Luminous Studio engine.
The resulting IgGs were detected by incubation with secondary antibodies conjugated to HRP, and developed using the Western Lighting reagent.
Bound antibodies were detected with horseradish peroxidase-conjugated secondary antibodies and developed using the Lumi-Light kit (Roche Applied Science).
Membranes were probed with the indicated primary antibodies and the appropriate HRP-conjugated secondary antibodies (Dianova) and developed using the ECL detection system (Amersham Pharmacia).
Following incubation, the plates were washed, probed with detection antibody and streptavidin-ALP and developed using the AP Conjugate Substrate Kit (Biorad).
The sections were washed in PBS to remove excess antibody and developed using the M.O.M. and peroxidase DAB kit, obtained from Vector Laboratories (Burlingame, CA).
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