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A fully automatic miniature surface plasmon resonance (SPR) concentration analyzer having high performance and low cost and developed using a Spreeta™ sensor was designed for field applications and concentration analysis.
This paper summarises the implementation of an industrial pilot plant with a malaxer machine designed and developed using a system that allows the control and the monitoring of malaxing parameters (CMPS).
A weed detecting robotic prototype is designed and developed using a Raspberry Pi micro controller and suitable input output subsystems such as cameras, small light sources and motors with power systems.
Autoradiograph film (Amersham) was exposed to the membrane and developed using a Kodak film processor.
The gel was then dried and exposed to phosphorimager plate and developed using a Typhoon phosphorimager.
The membranes were exposed overnight with intensifying screens and developed using a Molecular Imager FX (Biorad).
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The membranes were subsequently incubated with the indicated antibodies and developed using an enhanced chemiluminescence (ECL) method, as described by Fazal et al. [19].
The protein was transferred to a PVDF membrane, probed with anti-PW1 and anti-Hsp70 antibody, and developed using an ECL chemiluminescence kit (Pierce).
The membranes were incubated with a secondary antibody coupled to horseradish peroxidase and developed using an enhanced chemiluminescence system (ECL plus; Amersham Pharmacia Biotech).
Membranes were incubated for 1 min with the SuperSignal ECL reagent (Pierce) then exposed on BioMax Light Film (Kodak) and developed using an RP X-OMAT Processor (Kodak).
Membranes were washed with TTBS, incubated with horseradish peroxidase-conjugated species-appropriate secondary antibodies (Jackson Immunoresearch, West Grove, PA) for 1 hour at room temperature, and developed using an enhanced chemiluminescence system (Supersignal; Pierce, Rockford, IL).
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