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In [ 8] the authors start from a different viewpoint and determine, using a simulation model, how many elective cases can be performed in a dedicated orthopedic trauma OR.
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The proline content in each sample was measured in milligrams per gram of fresh weight (FW) and determined using a standard curve of analytical-grade proline.
The thickness of the films (around 100 μm) was taken as the distance between the electrodes and determined using a micrometre gauge.
Fluorescence was detected orthogonally to the excitation path and determined using a Jobin Yvon Triax 190 monochromator.
We used a phylogeny of Medicago species previously published, and determined using a different locus than the one used for testing positive selection.
Blood pressure was evaluated by the investigator after a 5-min resting period with the individual sitting in a chair and determined using a standard mercury sphygmomanometer.
Cell viability was determined using a trypan blue exclusion test and cell number determine using a haemocytometer.
After 6 h of induction, luciferase assay reagent was added and luminescence determined using a luminometer.
Nonphagocytosed apoptotic PMN or E. coli were washed off, extracellular fluorescence quenched, and phagocytosis determined using a fluorescence plate reader.
Cells were trypsinized and density determined using a Vi-Cell counter (Beckman Coulter Corp).
Neonatal length and weight were determined using a measuring board and a calibrated scale.
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