Fluorescence was excited using a 470 nm interference filter and detected using a 530 nm cutoff filter.
CFZ's MΦ-targeting capability, associated with the formation of unique intracellular crystallized inclusions (CLDIs), was confirmed via conventional immunofluorescence assays and detected using a PA microscope (Fig. 2).
The SFG signal was spectrally resolved and detected using a spectrograph (Acton Spectro Pro® SP-2300, Princetogetherruments) together with an emCCD camera (ProEM 1600, Roper Scientific).
Amplicons were then hybridized to the microarray and detected using a combination of signal amplification and fluorescence.
As model samples, dopamine and catechol were separated and detected using a permanganate chemiluminescent system on the prepared microchip.
The free vibration of the microcantilever was induced by an ultrasonic transducer and detected using a Michelson interferometer.
All PCR products were analyzed by electrophoresis on polyacrylamide gel (10 %) and detected using a silver staining method.
The PL was collected by an optical fiber and detected using a Horiba Triax 320 spectrograph coupled with an Andor spectroscopic CCD camera.
The luminescence from the sample was collected by the same objective lens, dispersed by a spectrometer, and detected using a charge-coupled device.
The PL signal was collected and detected using a spectrometer (HORIBA iHR 550) equipped with a liquid nitrogen-cooled InGaAs photodiode detector (1300 2300 nm).
PL was collected at normal incidence to the surface of samples through a fiber, dispersed in a spectrometer (Princeton Instruments, SpectraPro 300i), and detected using a Peltier-cooled charge-coupled device (CCD) camera (PIXIS 100F).
