Sentence examples for and detached with a from inspiring English sources

After PBS removal, cells were incubated with 1.8 mL sample buffer (8 M urea, 2 M thiourea) and detached with a cell scraper (Greiner BioOne).

For preparative cell sorting, cells were washed once with PBS and detached with a trypsin/EDTA solution (0.1% trypsin, 0.25% EDTA in PBS) at 37°C for 5 min. Cells were centrifuged at 1200 rpm for 5 min, washed again with PBS and re-suspended in sorting buffer (PBS, 2% BSA, 5 mM EDTA).

Cell mono-layers were washed with PBS and detached with Versene (supplied as a 1× solution of 0.2 g/l ethylenediaminetetraacetic acid, Life Technologies, Carlsbad, CA, USA) at 37°C.

After another 24 h, cells were washed with PBS and detached with trypsin.

Cells were first washed with PBS and detached with 0.2 mL of 0.25% trypsin.

Cells were then placed on ice, washed with ice-cold-PBS, and detached with trypsin.

Cultures were then washed extensively with RPMI and detached with cold PBS.

Cells were then washed with ice-cold PBS and detached with ice-cold PBS containing 5 mM EDTA.

24 h prior to the measurements, cells were washed with phosphate-buffered saline (PBS), and detached with trypsin-EDTA.

Cells were then washed twice with DPBS containing 200 µg/ml ciprofloxacin and detached with trypsin.

The cells were then washed with PBS and detached with trypsin at 37°C.