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In this report, we describe how we created a recombinant antibody that specifically recognizes EGFRvIII and demonstrate its specificity by western blot analysis, immunohistochemistry (IHC), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA) and flow cytometry analysis (FACS).
In human studies, T1rho continues to prove sensitive to early OA, but more work is required to understand and demonstrate its specificity for molecular changes to the proteoglycan and/or collagen components of articular cartilage [ 40].
To study this effect in more detail and demonstrate its specificity, we incubated TF-1 and ML-1 sublines overexpressing either PD-L1, PD-L2 or CD86 with corresponding antibodies blocking the interaction of these T-cell ligands with their receptors.
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RT-PCR analysis of mRNA isolated from these salivary glands showed a strong reduction in Ir-LBP mRNA level compared to the actin mRNA used as control (Figure 8B), confirming the results in the endogenous system and demonstrating its specificity.
To further demonstrate its specificity, we did Western blot on all neurotrophins.
Lanzmann (as Kael says) "accumulates data compulsively": it's both one of his methods for depicting the Holocaust and for demonstrating its specificity — as experience and as history.
Some studies have indicated that PD098059 failed to inhibit the stress, the stimulated JNK/SAPK and the p38 pathways, demonstrating its specificity for the ERK pathway (Alessi et al, 1995; Dudley et al, 1995).
Sixty-one specimens containing viruses or bacteria other than Legionellae were negative according to 23S-5S PCR, demonstrating its specificity.
In all cases, the binding was inhibited by RanQ69L-GTP, demonstrating its specificity.
This antibody did not recognize the mutant CypD-K166R, thus demonstrating its specificity.
We demonstrated this by immunohistochemistry using a polyclonal antibody after having clearly demonstrated its specificity for NBPF proteins.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com