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Following which, the area of interest in the tissue section was brought to focus at 20X magnification on the computer monitor connected to the microscope and delineated using a computer graphic tool.
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This, together with evidence of strong correspondence among the networks defined by iFC and those delineated using a variety of other neuroimaging techniques, suggests a fundamental brain architecture detectable across multiple functional and structural imaging modalities.
An averaged image of the last three frames of the scan was produced and lesions were delineated using a 50% threshold of the peak standardised uptake value (SUVpeak).
Briefly, inserts were amplified using the M13 forward primer, sequencing reactions were performed using BigDye Terminator v3.1 (Applied Biosystems), and sequences were delineated using a PRISM™ 3730xl DNA Analyzer (Applied Biosystems).
This appeared to be more effective when high and low vulnerability were delineated using a lower threshold, resulting in a more balanced dataset [ 32].
Noise artefacts were eliminated with the patient motion reduction algorithm and suspicious tissues were delineated using a novel all-timepoint-based, threshold-independent parametric map approach.
Scanning parameters were as follows: repetition time = 12 ms or 15 ms, echo time = 5.4 ms, and inversion time = 650 ms. Both baseline and repeat brain regions were delineated using a semiautomated technique based on intensity thresholding and mathematical morphological operations [38].
For each surgical cohort, patients with bleeding-related complications and/or blood product transfusions and those without these events were further delineated using a combination of diagnosis and procedure codes.
The tumour was then automatically delineated using a 42% SUVmax threshold, and this delineation was used to assess the tumour volume [15].
The unstained area of all serial striatal sections (400 µm apart) was manually delineated using a 4× objective and an AX70 microscope (Olympus, Munster, Germany) coupled with an image analysis system.
SEPs ranging from 0.0 to 0.78 were assigned to 176 unique ecoregions that were delineated using a combination of three mapped climate variables (Table 1) and existing forest cover (see Additional file 1: Appendix 1 for detailed methods).
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