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A single curCNVE could include both duplications and deletions of a particular region.
These are mostly insertions and deletions of a base pair that is identical to its neighbor resulting in no actual FS.
Non-frameshifting tandem duplication insertions and deletions of a repeated motif are always conservative events, irrespective of the phase they occur in.
The analysis of human genome next-generation sequencing (NGS) data largely focuses on the detection of single nucleotide variants (SNVs), and insertions and deletions of a few base pairs (indels).
In the seven mutants in which both ends of the insertion sites were characterized, deletions of genomic sequences (≤ 5 bp) were found in only three mutants (13_H5, 18_M24 and 1a7-4033, Additional file 5: Table S2), and deletions of a wide range of sizes, from 5 bp to 1.8 kb, were observed at ends of the insertion vectors in all mutants.
For example, using a stringent 3 consecutive probe criteria, deletions of ~100 bp require microarrays with probes staggered ≤ 45 bp while smaller deletions of ~30 bp need probes staggered ≤ 32 bp and deletions of a just a few bp require probes staggered ≤ 12 bp.
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β-glucans of the cell wall are involved in triggering innate immune responses against A. fumigatus [41], [42] and deletion of a β-1,3-glucanosyltransferase, GEL2, reduced virulence of A. fumigatus in a murine model [43].
In the developing nervous system, VEGF released by neuroblasts and glia induces the ingrowth of capillaries, and deletion of a single copy of VEGF is lethal [ 6, 7].
A circular doubly linked list has been utilized to implement the list of single bucket lists, thus ensuring the insertion and deletion of a bucket quickly.
10 2) the P-body assembly process is highly redundant and deletion of a single protein does not abolish the aggregation process completely.
In the TKO strain, MF decreased significantly (Table 1), and the only types of mutations observed were low levels of targeted G → T and 5′-C → T transversions and deletion of a T from the run of three T residues.
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