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The phrase "and definite knowledge of" is correct and usable in written English.
You can use it when discussing a clear and certain understanding of a subject or topic.
Example: "To excel in this field, one must have both practical experience and definite knowledge of the underlying principles."
Alternatives: "and clear understanding of" or "and certain awareness of".
Exact(1)
Therefore, the appropriate and definite knowledge of all the HBV genotypes prevalent in a certain region is of immense importance for the proper and effective management of HBV patients.
Similar(59)
The Gurungs – and to a lesser extent Magars – were also thought to be good at hunting and to have the most definite knowledge of, and penchant for, wild foods.
The small size of the corpus, the absence of bilingual texts, and the lack of definite knowledge of the underlying language has frustrated efforts at decipherment since the discovery of the remains of the Indus civilization.
They reported the Government had ducked an Official Secrets Act prosecution against him for revealing his most damming secrets - MI6's definite knowledge of and alleged complicity in a coup attempt in Libya, and MI5's lazy handling of warnings of the bombing of the Israeli Embassy which led to the building being wrecked and the probable unjust conviction of two Palestinians.
Obstacles to the decipherment of the sign system include the paucity of long texts, the absence of bilingual text, and the lack of any definite knowledge of the underlying language(s) the script may have expressed.
A little farther to the west, the new Globe rises up on the Bankside, asserting definite knowledge of William Shakespeare's theatre and deserving praise for doing so; but the difficulty of seeing the earlier theatres in the shadows of the past better represents our understanding of performance in Shakespeare's theatre.
Within a generation or two the Huguenots generally were as happy to forget their French origins as many other members of the land-owning and business classes were to forget their English; and by the early twentieth century no trace or definite knowledge of a former French connection remained.
However, without definite knowledge of Aboriginal/non-Aboriginal co-morbidity and cancer treatment differences, including adherence, these explanations remain plausible at best.
Advance agreement by all concerned is greatly superior to trying to reach the right decision in a crisis situation, without definite knowledge of the individual's preference.
But I was forced to recognize that this was utterly hopeless until I possessed certain definite knowledge of the controversial points.
"My first definite knowledge of a press section at a convention is at the 1868 Democratic National Convention held at Tammany Hall" on East 14th Street, Mr. Sautter said in an e-mail message.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com