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29Si was used for internal standardization and data were calibrated against two standard reference materials (NIST SRM 610, 612) and Corning B, C and D. The elemental compositions were then calculated according to Gratuze [38].
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Mössbauer spectra were collected at room temperature, 140, 77, and 13 K, and data was calibrated to a spectrum of α-Fe foil collected at room temperature.
PLS models based on the NIR data and UV data were calibrated and validated.
The fluorescence and transmittance data were calibrated and corrected (for possible system drift) based on the methods discussed in Sections 3.1 and 3.2.
Elongation factor 1-alpha (EF1a) was used as the reference gene, and the data were calibrated relative to the transcript levels in resistant trees prior to nematode infection (at 0 dpi).
In order to exclude the confounding effects by age and gender, the audiometric raw data were calibrated for the effects of age and gender on the basis of the Diagnostic Criteria of Occupational NIHL (Chinese National Criteria GBZ49-2007).
X-ray fluorescence data were calibrated and checked with the measurement of a 200-μm-thick cube of NIST National Institute of Standards and Technologyy) silicate glass certified reference material SRM 610 within each diamond anvil cell at ambient conditions.
All data were calibrated and annotated to the Mosaiques human urinary database [ 47].
Real-time PCR was conducted with the primers for PPARGC1A on the 4 samples of all 50 animals, after which the mRNA expression data were calibrated and converted into raw data in the same manner as described above for the reference genes.
All PET data were calibrated, normalised, and corrected for attenuation, random and scattered coincidences.
The obtained age data were calibrated using the OxCal 4.2 program (Ramsey 2009) and the calibration curve IntCal13 (Reimer et al. 2013).
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