Sentence examples for and cryopreserved in a from inspiring English sources

However, in the same study sterile protection was induced in mice immunized with a total of 6000 irradiated P. yoelii sporozoites (given in three equal doses) that were purified and cryopreserved in a similar manner to those from P. falciparum.

We obtained superior results in term of antral-like cavity formation, cumulus-oocyte complexes collection and MII collection than that from the report of dela Pena et al. [ 2], who cultured the thawed preantral follicles, vitrified and cryopreserved in a 0.25 ml straw, using the Transwell-COL membrane inserts method [ 19].

One technique has been published by Dunn et al. (1989) and Koebe et al. (1990), which demonstrated that rat hepatocytes that were cultured and cryopreserved in a sandwich gel culture were able to maintain their phenotypic properties and functionalities for a longer period of time than hepatocytes in suspension.

These human islets were isolated and cryopreserved in an era when many of today's modern techniques were undeveloped or prohibitive in terms of cost.

Cells were then counted using a Beckman Coulter viCell, and cryopreserved in freezing media (temperature lowered 1 oC per minute until -80 oC).

Mononuclear cells were isolated using a standard density gradient/centrifugation method with Histopaque and cryopreserved in liquid nitrogen.

PBMCs were isolated according to a standard, sucrose density gradient (Lymphoprep) protocol and cryopreserved in 10% dimethylsulfoxide (DMSO) in fetal calf serum (FCS) at −80°C until use.

These were retained and cryopreserved in 30% glycerol at −80 °C.

Prior to the start of the in vitro experiment, W20 17 cells were expanded and cryopreserved in multiple aliquots.

PBMC for longitudinal analyses were originally collected in heparin and cryopreserved in liquid nitrogen.

Peripheral blood mononuclear cells (PBMC) were separated and cryopreserved in liquid nitrogen until assay time.