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We found that canonical Notch signaling is required for late-stage epidermal differentiation and correct processing of filaggrin, a process when perturbed being closely associated with barrier function defects and skin disorders.
In addition, we created a C-terminal truncation construct (ΔIC) by removing the last 36 amino acids of the protein leaving the trans-membrane sequences intact rather than expressing only the soluble ectodomain of APL-1, attempting to maintain proper membrane anchoring and correct processing of the protein.
Previous studies suggest that such a functional node is likely the stochiometric activity of TDP-43 in ribonucleoprotein complexes that are essential for the regulation and correct processing of multiple target genes, including those related to synaptic function (15, 17).
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(2) to avoid and correct processing errors in process (Secured).
to avoid and correct processing errors in process (Secured).
A number of clones displayed green color on plates and this indicated correct processing of the signal sequence.
Stem-loop binding protein (SLBP) directly binds the stem-loop of histone mRNAs and is necessary for correct processing of histone pre-mRNAs [20], and is absolutely required for processing Drosophila pre-mRNAs in vitro [21].
Fundamental aspects of effective screening, such as the correct processing of the instruments and tests and correct specifying signs to intervene, were not performed well in the present study.
Epigenetic regulation of gene expression is necessary for the correct processing of developmental programs and the maintenance of cell fates.
This feature is extremely important for the automation of the methodology and the specialist knowledge is unnecessary to correct processing of the data.
This strategy promised not only the correct processing of both viral Npro and C protein but also releasing of the chimeric protein from the nascent viral polyprotein.
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