All statistical analyses were conducted in R. 30 The phylogenetic analysis included 80 sequences (Table 1 and Figure 2) and was conducted by using MEGA5.
All two sample tests used are Wilcoxon nonparametric tests and are conducted by using R language [ 36].
We have described results of a comprehensive, staged molecular analysis of unexplained encephalitis and meningitis conducted by using CFS samples obtained over a 3-year period in a major referral hospital in Taiwan.
PCR for detecting HRV and enterovirus was conducted by using EVP4 and OL68-1 primers, which detected HRV and human enterovirus, respectively, in amplicons of ≈530 and 650 bp, respectively (7 ).
Adapter ligation and DNA cluster preparation were performed, and sequencing was conducted by using an Illumina Hiseq2000 instrument according to the manufacturer's instructions (Illumina, San Diego, CA).
All regressions and statistics were conducted by using GraphPad Prism version 5.03 for Windows (GraphPad Software, San Diego, CA, USA), and a P value of less than 0.05 was considered statistically significant.
Bacterial detection and identification were conducted by using molecular methods based on real-time PCR, classical PCR, and nucleotide sequencing (Table).
As comparison, the eleventh and twelfth tests were conducted by using 50%% and all of the non-landslide pixels respectively and the variables in equation were produced in SPSS.
To construct the model, training and testing was conducted by using experimental results from 82 specimens.
An experimental program that contains 21 different combinations of independent parameters (CT, CTime, SC and SM) was conducted by using the central composite design method.
