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The absorbance values and concentrations of each cytokine were determined using a Ceres 900 automated microplate reader (Bio-Tek Corp., Wisnooski, VT, USA) and Kineticalc software (Bio-Tek).
Associations between admission MPV, ΔMPV24h, admission platelet count and ΔPlat24h in the whole sample and concentrations of each of the measured cytokines were evaluated by linear regression.
When the velocity term is considered, and hence the interface kinetics is taken into account, the dynamical Gibbs-Thompson relation states that the chemical potentials and concentrations of each species deviate from their equilibrium thermodynamic values at the interface.
The spectral emission differed for all seven Caribbean coral species (fig. 2) indicating inter-specific variation in the presence and concentrations of each FP.
To establish the 50% inhibition concentration (IC50) of each antibody, cells were incubated (16 h, 37°C) in duplicate wells with 0.25 1500 nM antibody and concentrations of each toxin producing 50% maximum T-cell stimulation: SEB (0.35 nM), SEC1 (1.3 nM), or SpeC (467 nM).
The image on the left is what the array looks like and on the right is the layout of the locations, relative proportions and concentrations of each of the dyes.
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technique, it was possible to study the effects of both molecular weight and concentration of each component.
Then ten micro liters of sample solution was injected and concentration of each component was determined through the calibration graphs of the respective standards.
Whole-cell computational models aim to predict cellular phenotypes from genotype by representing the entire genome, the structure and concentration of each molecular species, each molecular interaction, and the extracellular environment.
To ensure the integrity of the RNA prior to use, the quality and concentration of each sample was tested using an Agilent 2100 Bioanalyzer.
Both Mia.pp32 lines and Mia.EV proliferated at the same rate, as indicated by negligible differences observed in cell surivival percentages between the cell lines at extreme low doses and concentration of each drug tested (Figures 2A-C).
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