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These fractions were combined and concentrated with a Centricon Concentrator 10 (Millipore, Billerica, MA) to about 5 mL.
The supernatant was collected and concentrated with viva-spin column (100 kDa cut-off) ensuring the concentration of higher-molecular weight structures.
Purified gp41-MinTT protein was dialyzed against PBS and concentrated with a 3 kDa Amicon Ultra-15 device (Milipore) to reduce SDS concentration to <0.1%.
Domaine des Genèves Vaucoupin $30 **½ Well balanced, crisp and concentrated with citrus flavors.
This is firm and concentrated with considerable depth and rich, chocolatey tannins.
Éamon was a superb actor, focused and concentrated, with a powerful stage presence even when silent.
(Dreyfus, Ashby & Company, New York) Frédéric Magnien Chambolle-Musigny Vieilles Vignes 2003 $53 ** Rich and concentrated with plenty of toasty oak and ripe fruit.
The lentiviral particles were harvested at 48 and 72 h after transfection and concentrated with XE-90 Super Speed Centrifuger (BECKMAN).
These fractions were merged and concentrated with Amicon Stirred Cell 50 ml MWCO 1k (Millipore) to 100 μM for NMR-use.
These extracts were collected and concentrated with a Rotavapor at 40°C.
The fractions eluted with 50 and 100 mM imidazole were pooled and concentrated with an Amicon Ultra-15 instrument (Millipore).
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