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All groups were fed diets containing roughage and concentrate in a 70 30 ratio.
In addition, in cavernous areas of the seabed, sounds could bounce off the walls and concentrate in a smaller area.
The effects of feeding different levels of forages and concentrate, in a discontinuous feeding pattern, on the efficiency of feed utilisation and rumen function were studied using rumen fistulated sheep.
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The eluted protein was buffer-exchanged into buffer C (0.1% DDM, 20 mM Tris, pH 7.5) and concentrated in a concentrator with a 100 kDa cut-off membrane (Millipore).
The filtered extracts were combined and concentrated in a vacuum concentrator.
PCR replicates of each target gene were pooled, purified using GFX PCR DNA and Gel Band Purification kit (GE Healthcare), according to the manufacturer's protocol, and concentrated in a speed vacuum concentrator 5301 Eppendorf, A-2-VC rotor.
Cells were harvested at exponential phase (turbidity525nm = 0.40 0.45) by centrifugation, disrupted with a sonicator and concentrated in a speed-vac concentrator.
The column was then washed with 10 bed volumes of buffer B and eluted with 10 mM NADP+ in buffer B. Colored fractions were pooled and concentrated in a 30000 molecular weight cutoff (MWCO) Amicon concentrator at 4 °C.
But this could help Europe more than America, whose exports to China have been weak and concentrated in a few categories like aircraft, turbines and soybeans, while European companies have been more successful in selling high-end consumer goods there.
On the other hand, the use of membranes permits the contaminants to be isolated and concentrated in a separate compartment.
The colloidal material produced in this fashion is continuously captured and concentrated in a fixed bed filter located within the spouted vessel reservoir wherein arsenic complexation occurs.
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