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In severe deformities, gradual correction prior to surgery can be achieved with circular external fixation, but the assemblies are difficult and complex to perform, thus limiting its application, particularly with small feet.
Traditional peptide purification procedures have many disadvantages; they are lengthy and complex to perform, exhibit a low recovery of desired products and a loss of activity (Kandula and Terli 2013; Goh and Philip 2015; Panagiota et al. 2016; Wang et al. 2016).
Additionally, like all personalized regimens, it is more difficult and complex to perform.
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Two or more proteins can bind together and form a complex to perform various tasks; and solving the structures of these complexes can be challenging, even if the structures of the protein subunits are known.
Urine electron microscopic detection of viral aggregation has been reported to be highly sensitive and specific but is complex to perform and not used routinely [ 15].
Due to the intrinsic characteristics of PTH physiological secretion with very frequent and small pulses, PTH pulsatility studies are complex to perform and this explains in part the lack of published data on this topic.
While we would like to have performed similar migration experiments using normal and SSc-ILD fibrocytes produced by differentiation of monocytes in vitro, our current observations identified differences between the starting normal and SSc PBMC populations that made these experiments too complex to perform and interpret as part of the current study.
It is possible that MrkH and MrkI form a protein complex to perform the regulatory function.
However, these studies are complex to perform and susceptible for biases.
The IHA is complex to perform and should only be undertaken by experienced laboratory personnel.
Furthermore, the FPNI-PCR method is completed in a shorter time-scale than other widely-used genome-walking techniques, and is significantly less technically complex to perform.
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