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Mutants were characterised and compared with wild type protein.
The best Mut-A89N/F154Y was obtained and compared with wild type RtSCR9, the Vmax and kcat of Mut-A89N/F154Y were enhanced by 2.08 and 3.86-fold, respectively, while the Km decreased by 2.36-fold.
Laboratory-born samples of each population were tested on their beach of origin and compared with wild individuals of the same age; other laboratory-born samples were tested on both beaches, that of origin and the other beach.
Production of curdlan by mutant M5 strain in bioreactor was studied and compared with wild strain (Figure2).
Figure4 shows the time profiles of cell concentration, ammonium, phosphate, sucrose consumption and curdlan production by mutant M5 and compared with wild strain.
The digestion pattern was analyzed and compared with wild type MD1BAC.
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Intact suberin in native periderm from potatoes that are genetically modified to alter the biosynthesis of specific suberin structures (Table 1; see Table S1 for comparative chemical composition of depolymerization products) was analyzed and compared with wild-type native and wound periderms.
Sequences were analysed and compared with wild-type samples and a reference sequences using BioEdit software (Ibis Biosciences, Carlsbad, CA, USA) and Ensembl and NCBI databases.
The mutant proteins were expressed in Escherichia coli, purified, and compared with wild-type PA in various assays.
The activity of these mutagenized promoters was tested in HeLa cells and compared with wild-type constructs.
Heterozygous ΔKPQ- SCN5A knock-in TG mice were characterized and compared with wild-type (WT) littermate mice.
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