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Samples were run according to the LC timetable indicated by the column manufacturer and collected using the Gilson FC203B fraction collector.
cRNA was synthesized from linear cDNA using the mMessage mMachine T7 Ultra transcription kit (Ambion, Austin, TX) and collected using the RNeasy micro kit (Qiagen, Valencia, CA) at a concentration ranging from 500 to 1500 ng/µL.
These data were recorded and collected using the same methods for the BRCA1 and BRCA2 families.
A549 cells were prepared and collected using the same procedure as described above.
Samples were run through using the LSRFortessa, and analyses were performed and collected using the BD FACSDiva software.
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Reactions were run and data collected using the Opticon™ 2 system (Bio-Rad, Hercules, CA).
Quantitative real-time PCR (qRT-PCR) was performed using the ABIprism 7000 and data collected using the 7000 System SDS Software Applied BiosystemsSDS Software Applied Biosystems
Stimuli were generated and responses collected using the Psychophysics Toolbox running under Matlab 5.2 on a G4 Macintosh computer (OS 9.2).
Images were processed and data collected using the ArrayVision (Imaging Research Inc).
Therefore, data on weight and height collected using the questionnaire was considered to be pretreatment data.
The experiment was programmed (and data were collected) using the software package E-Prime.
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