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Mice were perfused with cold PBS and primary tumors were removed and chopped with a McIlwain Tissue Chopper (Mickle Laboratory Engineering Company).
Briefly, number 4 and 5 glands were excised and chopped with a razor blade, digested with 0.2% trypsin and 0.2% collagenase A and cells and organoids were enriched by differential centrifugation.
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He's a demented savant scribe, preaching, moaning, orating, and chopping with a guttural wolf-whisper.
Bisected tissue from the fifth true leaf of adult plants were harvested (see above) and immersed in magnesium sulfate buffer [ 37] and chopped with razor blades in a petri dish.
Chopping buffer (1.5 ml) was added to the dish, and leaves chopped with a razor blade, mixing until a paste was formed, 2 to 4 minutes.
The peel comes off easily with a vegetable peeler, and it can be chopped with an ordinary chef's knife.
These can be placed in glasses with milk and chopped up with a fork or just thrown into a blender.
The cortices were collected in a sterile beaker containing MEM/HEPES medium (25 mM HEPES and 10% v/v FBS and 100 U/100 μg/ml penicillin/streptomycin) and chopped up with a sterile scalpel.
The Agriculture Department-recommended safe minimum cooking temperatures are: 145 degrees for steaks and chops (with a three-minute rest time), 160 degrees for ground meat, 160 degrees for poultry and 145 degrees for fish.
For steak, chicken and chops with a great view of Waterpocket Fold; $20 to $25 per person.
Place your thyme on a board and chop with a large knife.
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