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Relative expression levels were normalized to GAPDH and calculated using the 2−ΔΔCt method.

The genomic features (promoter, exon, intron, 3′ untranslated region and intergenic regions) were defined and calculated using Refseq and HOMER.

Focal adhesion (FA) characteristics, including the location and FI, were detected and calculated using our customized software developed in MATLAB based on the water algorithm.

P-values represent gene-based burden tests for rare variants in PLD3 (MAF<5%) based on variant counts (Supplementary Table S1) and calculated using χ2 test statistics.

The output voltages measured in experiments and calculated using the above models agree well.

The radiation characteristics of the transmitarray are simulated and calculated using the finite integral technique (FIT).

The theoretical values of the linear-attenuation were extracted from the mass-attenuation coefficient and calculated using the XCOM program.

Individual lag phases of about 100 individual cells per condition were examined and calculated using a linear extrapolation method.

Chemical shift perturbations were calculated using previously published GR DBD NMR chemical shifts14 and calculated using the minimum chemical shift perturbation procedure69 in the NMR analysis program NMRViewJ (OneMoon Scientific, Inc.).

All data were normalized by GAPDH transcript and calculated using the ∆∆Cq method.

This is represented as a variable r and calculated using Eq. 4.

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