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Six rhesus monkeys were primed intramuscularly with IDLV-Env and boosted with the same vector after 1 year.
Mice were primed and boosted with the two vaccine vehicles by different routes and in different combinations.
Therefore, serum collected from mice primed and boosted with the recombinant proteins were tested for HAI titers.
CD4 T cells from donors primed by BCG and boosted with the vaccine similarly transferred protective immunity.
Five groups of mice were immunized with different amounts of the inactivated particles formulated with 300 µg Alum and boosted with the same dosage after 2 weeks.
In group 4, three pigs (numbers 12 to 14) were vaccinated with BEI-inactivated C-S8p260 with adjuvant, and boosted with the same dose and composition of antigen at 15 days post-immunization.
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Furthermore, the highest levels of lymphocyte proliferation response, IL-4, IL-12 and IFN-γ production were induced following priming with the DNA vaccine and boosting with the rgD protein.
Both priming with the adenovirus or recombinant multi-antigen and boosting with the modified vaccinia Ankara vector achieved a high degree of activation of TNFα and IFNγ-secreting CD4+ and CD8+ specific T cells 60 days after the first immunization.
We found that priming with the polyepitope construct and boosting with the mixture of peptide in A2-transgenic mice resulted in: (1) CTL responses not only against the peptide-sensitized T2 and SW480 cell lines but also the non-sensitized reconstructed B16 cell line; (2) expression of HLA-A*0201/H-2Kb chimeric gene and polyepitopes by B16 led to its rejection by immunized A2-transgenic mice.
We confirmed their observation of autoantibody responses following immunization and boosting with the same peptide antigen and extended the work by immunizing with a peptide from rabbit N-methyl-D-aspartate receptor NR2b (GR) [5].
Interestingly, the use of an suboptimal mimotope for primary vaccination and boosting with the wild-type epitope led to higher amount of AH1-specific T cells which have increased affinity and decreased off-rate for AH1.
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