Sentence examples for and blocking in a from inspiring English sources
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Tissues were permeabilized and blocked in a single step using a solution of 0.1% Triton-X100 in PBS, with 10% donkey serum.
On the day cells were stained, they were washed with PBS and blocked in a mixture of 0.5% BSA, 2 mM EDTA, and FcR blocking reagent (Miltenyi, Auburn, CA) for 20 minutes.
Select tissue sections were incubated for 1 h at RT in 2 N HCl, extensively rinsed with borate buffer (pH 8.5), and blocked in a solution containing 10% BSA, 10% goat serum in Tris buffer for 1 h.
paraformaldehyde, washed three times with PBS, and blocked in a solution of 5% (vol./vol)./vol
After electrophoresis, proteins were transferred onto nitrocellulose membranes and blocked in a 5% nonfat dried milk solution at room temperature.
The strips were thereafter washed in PBS buffer containing 0.01% Tween 20 and blocked in a PBS buffer containing bovine serum albumin.
The cells were permeabilised and blocked in a one-step procedure with 10% normal goat serum (NGS, Sigma) and 0.1% TritonX-100 (NBS Biologicals) in PBS for one hour at room temperature prior to immunostaining.
The sections were then washed 3 times in 0.1 M PBS for 5 min and blocked in a 0.1 M PBS solution containing 5% goat serum and 1% Triton-X-100, for 1 h at room temperature.
Proteins were then transferred on to a methanol-activated Immobylon™ PVDF membrane and blocked in a 5% (w/v) non-fat dried skimmed milk powder, PBS solution for 2 h at room temperature.
