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Tissues were permeabilized and blocked in a single step using a solution of 0.1% Triton-X100 in PBS, with 10% donkey serum.
Select tissue sections were incubated for 1 h at RT in 2 N HCl, extensively rinsed with borate buffer (pH 8.5), and blocked in a solution containing 10% BSA, 10% goat serum in Tris buffer for 1 h.
On the day cells were stained, they were washed with PBS and blocked in a mixture of 0.5% BSA, 2 mM EDTA, and FcR blocking reagent (Miltenyi, Auburn, CA) for 20 minutes.
Bead arrays were washed and blocked in a rocking incubator.
paraformaldehyde, washed three times with PBS, and blocked in a solution of 5% (vol./vol)./vol
After electrophoresis, proteins were transferred onto nitrocellulose membranes and blocked in a 5% nonfat dried milk solution at room temperature.
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Any actor can learn all the lines and blocking in a single weekend.
Our consumer service is censored and blocked in mainland China today," a spokesperson told TechCrunch via email.
These hemispheres were stored in 10% sucrose in PBS at 4°C overnight and were blocked in a solution of 11%gelatin/10%% sucrose.
"YouTube is blocked in about 80% of U.S. K-12 institutions, and it's blocked in a lot of corporations too…it isn't perceived as a safe place to send kids to learn," he says.
From stark neon-green and black blocking in a control room sequence, to dynamic outlining in a courtroom scene, the colors in this issue are subtle but breathtaking.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com