The phrase "and assigned using the" is correct and usable in written English.
It can be used in contexts where you are describing a process of allocation or distribution that involves a specific method or tool.
Example: "The tasks were organized and assigned using the project management software."
Alternatives: "and allocated through the" or "and designated via the".
Processed spectra were analysed and assigned using the program SPARKY (http://www.cgl.ucsf.edu/home/sparky/).ucsf.edu/home/sparky/
The statistical parameters for the intermediate hypothesis or conclusion nodes are defined and assigned using the PoI.
Exact masses were calculated for UDP-GlcNAc and its isotopologues and assigned using the program PREMISE as previously described [ 5].
Spectra recorded at 700 and 750 MHz were processed, displayed, and assigned using the NMRPipe software package.
Minimum convex polygons (95%) were chosen to delineate the borders of the subpopulations and assigned using the Home Range Tools (Rodgers et al. 2007) extension in ArcGIS (Redlands, CA, USA).
Then, the statistical parameters for the intermediate hypothesis nodes are defined and assigned using the principle of indifference (PoI) [ 34], which states that " each member of a set of propositions could be assigned the same probability of truth in the absence of any reason to assign them different probabilities".
Data were analyzed (and significance assigned) using the ratio t-test in the GraphPad Prism 4 software.
Sequencing reads were functionally and taxonomically assigned using the Burrows-Wheeler alignment tool (bwa) [ 13] against the Human Microbiome Project database.
Diagnosis of every specimen was performed by a breast pathologist from hematoxylin and eosin (H&E) stained slides; staging was performed using guidelines defined by the AJCC Cancer Staging Manual seventh edition [ 11] and grade assigned using the Nottingham Histologic Score [ 12, 13].
Briefly, the images were manually manipulated when necessary to adjust the local threshold and chromocenter area and number were assigned using the Analyze Particle function of the software.
Pathways and enzymes were assigned using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database options built into the pipeline.
