Sentence examples for and assessed expression of from inspiring English sources
We assessed point mutations in K- ras and p53 genes in tissue samples by polymerase chain reaction/single-strand conformation polymorphism and assessed expression of p53 protein by immunohistochemical methods.
To confirm our impression that the disruption of AHDC1 was an unlikely cause of the congenital cardiac disease in DGAP105, we turned to the mouse and assessed expression of Ahdc1 and Matr3 during stages of mouse embryonic development that correspond to times at which the relevant developmental timing and tissue interactions for LVOT development occur.
To determine whether Tregs release cytotoxic molecules including granzymes and perforin upon activation, we stimulated T cells either with conventional αCD3/CD28-coated beads or with bsAb in the presence of target cells and assessed expression of the activation marker CD69 and degranulation marker CD107a.
This study was designed to identify the global profile of genes induced by heme in the endothelium, and assess expression of the heme-inducible cytoprotective enzymes in major organs impacted by SCD.
Moreover, we extracted RNA from MCF10A cells expressing an empty vector, HER2, HER3 or HER2/HER3 that were grown in three-dimensional cultures for 15 days and assessed expression levels of epithelial-to-mesenchymal transition (EMT) markers.
Having investigated mucus quantity by evaluating goblet cell number and mucin synthesis, we assessed expression of genes encoding host enzymes and mucus quality by studying the profile of mucin O-glycosylation.
To further compare the angiogenic and invasive phenotypes, we assessed expression of the pro-angiogenic factors VEGFA, ANGPT1, ANGPT2 and bFGF.
In addition, we assessed expression of MtSCP1, and MtBCP1, two genes expressed in this cell type that are suitable as additional markers.
As a previous report showed that moderate increase in mRNA levels of Mdr1a and Mdr1b in murine breast cancer can cause Dox resistance (22), we assessed expression of Mdr1a and Mdr1b in immortalized Mdr2 −/− MEFs by qPCR.
We assessed expression of XRCC3 and KU80 proteins on 60% confluent cultures by Western blot of nuclear and cytoplasmic fractions.
We further assessed expression of EMT and motility-related genes seven days after Runx2 induction in MCF7/Rx2dox cells.
