Sentence examples for and assays using the from inspiring English sources

These assays include electroimmunoassay [ 1], single radial immunodiffusion [ 2], slide reversed passive latex agglutination [ 13], non-competitive chemiluminescence enzyme immunoassay [ 3], ELISA [ 14] and assays using the latex agglutination immunoturbidometric principle [ 15, 16].

Peak viraemia levels for each species are shown as relative plaque-forming units (pfu; bars represent means), estimated by extrapolation from a standard curve generated using serial dilutions of RNA isolated from the challenge virus and assayed using the same method as the test samples26 (b, sheep; e, goats; h, cattle).

Cells were harvested after two days and assayed using the Dual-Glo Luciferase Assay System (Promega) to determine the relative luciferase activity.

Human influenza A virus (A/PR/8/34 (H1N1)), obtained from Life Technologies Co., was used and assayed using the fifty-percent tissue culture infectious dose (TCID50) method.

Cell culture supernatants or BALFs were thawed and assayed using the BD™ Cytometric Bead Array (CBA) Mouse Inflammation kit (BD Biosciences, San Diego, CA) or by ELISA BDD OptEIATM, BD Biosciences, San Diego, CA) according to the manufacturers' protocols.

The cells were lysed and assayed using the dual luciferase reporter assay system (Promega).

Reaction products were diluted 1 3 with water and assayed using the Absolute Blue qPCR SYBR Green kit (ThermoFisher AB-4166BB).

Cells were lysed and assayed using the Dual-Luciferase Reporter Assay SysteMadisonega, Madison, WI).

Cells were harvested after 48 h, lysed and assayed using the dual luciferase reporter system (Promega).

For qRT PCR, cDNA was prepared using the RNEasy protocol (Qiagen) and assayed using the SYBR green detection system (Qiagen).

Cell lysates were prepared and AMPK immunoprecipitated and assayed using the SAMS peptide as previously described [ 28].