Sentence examples for and assayed utilizing a from inspiring English sources

Four different types of open-circuit processes have been designed and assayed utilizing a pilot plant comprising two hammermills, one screener and one dynamic air separator, in order to obtain the desired fineness for the product.

After 48 hours, the cells were harvested and subjected to lysis, and the luciferase activity was assayed utilizing a Dual Luciferase Reporter Assay System (Promega) according to the manufacturer's instructions.

To test this, we performed strand annealing assays utilizing a 5'-labeled single-stranded DNA substrate and a complimentary strand in the absence of ATP.

Both assays utilize a luciferase based reporter whereby protein expression is monitored by the amount of luciferase protein product.

High-throughput reporter assays utilize a 384-well format that enable the functional measure of thousands of endogenous human promoters.

All the assays utilized an epMotion® 5075 automated pipetting system (Eppendorf) and were performed at pH 5,0 with 50 mM citrate buffer.

After treatment for 3 and 7 days, cell viability was assayed utilizing the CCK-8 method.

Luciferase reporter assays utilizing ATF2 and a cAMP response element reporter demonstrated an increase in reporter activity under ER stress conditions, suggesting increased ATF2 phosphorylation.

Kv12.2 channel membrane expression was assessed by membrane surface biotinylation assay utilizing a 'Cell surface protein isolation kit' (Pierce, IL), according to instructions from the manufacturer.

Cycling and MTOC regrowth assays utilizing siRNAs were conducted 2 days after siRNA transfection.

Calpain cleavage assays utilizing cytoplasmic and nuclear lysates were performed as described above.