The phrase "and assayed using the" is correct and usable in written English.
It can be used in scientific or technical contexts when discussing the analysis or examination of a substance or material.
Example: "The samples were collected and assayed using the latest analytical techniques to determine their composition."
Alternatives: "and analyzed with the" or "and tested using the".
Peak viraemia levels for each species are shown as relative plaque-forming units (pfu; bars represent means), estimated by extrapolation from a standard curve generated using serial dilutions of RNA isolated from the challenge virus and assayed using the same method as the test samples26 (b, sheep; e, goats; h, cattle).
Human influenza A virus (A/PR/8/34 (H1N1)), obtained from Life Technologies Co., was used and assayed using the fifty-percent tissue culture infectious dose (TCID50) method.
Cells were harvested after two days and assayed using the Dual-Glo Luciferase Assay System (Promega) to determine the relative luciferase activity.
Cell culture supernatants or BALFs were thawed and assayed using the BD™ Cytometric Bead Array (CBA) Mouse Inflammation kit (BD Biosciences, San Diego, CA) or by ELISA BDD OptEIATM, BD Biosciences, San Diego, CA) according to the manufacturers' protocols.
Thawed supernatants were transferred to MultiScreen filter plates (Millipore; Billerica, MA) and assayed using the human cytokine/chemokine Milliplex™ MAP Kit 96-well plate assay (Millipore) using a Luminex100 IS System (Luminex Corp; Austin, TX) to determine the quantities of pro (IFN-γ, IL-1β, TNF-α) and anti-inflammatory (IL-4, IL-10 and IL-13) cytokines.
Cells were lysed and assayed using the Dual-Luciferase Reporter Assay SysteMadisonega, Madison, WI).
These assays include electroimmunoassay [ 1], single radial immunodiffusion [ 2], slide reversed passive latex agglutination [ 13], non-competitive chemiluminescence enzyme immunoassay [ 3], ELISA [ 14] and assays using the latex agglutination immunoturbidometric principle [ 15, 16].
Serum levels of zonulin and EGF were assayed using the ELISA kits (Immunodiagnostik, Bensheim, Germany and Boster Biological Technology, Fremont, USA, respectively).
AGPase, SS and GBSSI were assayed using the methods of Nishi et al. (2001).
48 h after transfection, cells were collected and luciferase activities assayed using the Dual Luciferase Kit from Promega.
Plasma cortisol and DHEA were assayed using the Correlate-EIA kits from Assay Designs (Ann Arbor, MI, USA).
