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In this review, we summarize several major fast calcium signaling pathways and discuss the recent developments and application of genetically encoded calcium indicators to detect these pathways.
To meet the challenge, the generation and application of genetically modified plants is justified.
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In this review, we describe recent advances in design and applications of genetically-encoded voltage indicators (GEVIs).
In this review, we chronicle the development and applications of genetically-encoded voltage indicators (GEVIs) over the past two decades, with a primary focus on the structural design that harness the power of fluctuating transmembrane electric fields.
The development and subsequent application of genetically encoded Ca2+ indicators based on fluorescent proteins (FPs) has revolutionized the study of intracellular Ca2+ dynamics.
We describe the design, characterization and application of a new genetically encoded fluorescent biosensor for intracellular detection of both free Ca2+-calmodulin and apocalmodulin, which together comprise the available calmodulin concentration.
Here, we report the construction and application of the first genetically encoded FRET-based biosensor for oxygen named FluBO which consists of the oxygen-insensitive FbFP donor domain and the hypoxia-sensitive enhanced yellow fluorescent protein (YFP) acceptor domain.
The rapid development of genetic engineering and extensive applications of genetically engineered (GE) animals have provided many research benefits, but concerns have been raised over the potential environmental impact of transgenic animals.
Before industrial application of genetically engineered bacteria and yeasts, several steps have to be taken.
The Cre8 portal, created in the context of the CREATE consortium, aims to integrate information from European and International Cre driver strains, through a virtual international application of genetically modified mice expressing Cre recombinase (6).
To date, there has been almost no application of genetically modified (GM) technology in commercial winemaking (Chambers and Pretorius 2010; Pretorius et al. 2012); therefore, non-GM strategies to develop flavour-active yeast are required.
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