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Then the supernatant was removed and an equal volume of DMSO was added to dissolve the formazan crystals.
The lipofection was conducted in OptiMEM media without serum, and an equal volume of 2× growth media was added to the cells after 4 h of lipofection to minimize cytotoxicity.
Briefly, pelleted fungal cells were resuspended in 30 ml by cold PBS, decanted into cold, sterile canisters and an equal volume of zirconium grinding beads, and centrifuged (550 r.p.m., 5 min, 3 cycles).
Transfection was conducted in OptiMEM media without serum, and an equal volume of 2× growth media was added to the cells after 4 h of lipofection to minimize cytotoxicity.
The supernatant (>90% radioactivity recovered) was collected, and an equal volume of MeCN was added.
Ten mL of air was taken out from the bottle and an equal volume of acetylene was injected.
At desired time intervals, 2.0 mL of release medium was taken out, and an equal volume of PBS was replenished.
The chloroform extract was then reduced in volume and an equal volume of diethyl ether was added, to get a red precipitate.
Mice were then retransfused with their shed blood and an equal volume of Ringer Lactate and were observed between the 120th and the 165th minute.
The blood samples were aliquoted in 2 ml Eppendorf tubes and an equal volume of CLPA reaction buffer was added (1 1).
Next, 40 µl 10% SDS and an equal volume of phenol/chloroforme/isoamylalcohol was added.
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CEO of Professional Science Editing for Scientists @ prosciediting.com