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The injection sites were sealed with melted dental wax, and the animals were returned to the rearing room and allowed to develop to the desired stage.
Synchronized new hatched first-instar larvae were placed on standard medium supplemented with yeast at 25°C and allowed to develop to the desired time points.
For developmental Western blots, Drosophila embryos were collected every 4 hr in large culture cages and allowed to develop to the appropriate stage.
Arrested L1 animals were transferred to nematode growth media plates with OP50 E. coli and allowed to develop to the L2/L3 molt (24 hr).
To test whether this ssODN mediated 794 bp chromosomal deletions was heritable, about 3000 silkworms from 11 G1 broods were reared and allowed to develop to the 3rd instar.
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Embryos were then allowed to develop to early blastula stages.
These embryos were allowed to develop to 4-6 hpf.
Embryos were allowed to develop in the absence of doxycyclin.
Rubin is likely the prospect who will be allowed to develop the most this season.
Adults were removed and embryos were allowed to develop.
For decades, the Brotherhood was never allowed to develop a stable role in Egyptian political life.
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