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The sample collected on May 3 and all further samples of this animal tested positive by BTV ELISA as well as by BTV real-time reverse transcription PCR (RT-PCR).
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DEK extracted DNA of samples from Greece, whereas IO and KEK carried all further sample and data analysis steps of all samples.
All further sample processing steps were carried out as described previously [ 39].
Another specimen discovered in Alaska was spotted in the collection of the Natural History Museum of Los Angeles County, and a further sample was obtained from tissue taken from a whale stranded on Unalaska Island in the Aleutians in 2004.
During follow-up assessments, participants underwent repeat assessment of symptoms and submitted further samples for sputum smear and TB culture.
A sputum sample may be taken for diagnostic tests, and further samples requested.
Samples of portal blood, bile and further samples of peripheral blood were taken intraoperatively.
After quality control, six microarray samples were removed from the original 72 samples and all further analysis were done on the remaining 66 microarray samples.
This filtering and all further analyses excluded four samples (noted in Appendix 1).
In all these habitats, recruitment of Cystoseira was virtually nil (see Results), and no further sampling was carried out.
We then randomly selected 50 samples for each of the tissues, and all further analysis was performed on these samples (Supplementary File 1).
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