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We predicted the UGT1A1 secondary structure profile by using the neural network programs PSIPRED [ 75] and NNPREDICT [ 76], and aligned it to the structural alignment of known bacterial and plant GT-B crystal structures by using hidden Markov models (HMM) with manual adjustments [ 77].
We translated each ORF sequence into its corresponding protein and aligned it to three protein databases — Refseq, human-nr, and full-nr — in an iterative, subtractive fashion using BLASTP (see Methods).
Wei et al. [ 19] generated a sequence-ready physical map of maize and aligned it to the genome of rice, revealing its complex evolutionary history.
Thus, we re-sequenced the gene hp1018 and aligned it to the published genomic data of H. pylori Hp26695 (Figure 3).
Since the BAC clone for HINT1 gene, 033L23, likely contains the breakpoint of the inversion, we sequenced the entire BAC and aligned it to the published zebra finch genome sequence (Fig. 5; Ensembl: www.ensembl.org).org
To transform the version 2 reference genome positions of the SNPs to reference version 3 positions, we took the ∼100 bp centered on the SNP and aligned it to the genome using BWA-MEM (default parameters).
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Performing the 3D reconstruction, importing it to the magnetic navigation system, and aligning it to the fluoroscopic views required only a few minutes and did not delay the procedure significantly.
The quality of the reconstruction was assessed by two operators performing the procedure and graded as (i) not possible to be reconstructed, (ii) poor, or (iii) good, after superimposing and aligning it to the real-time angiography in the LAO, AP, and RAO projections.
Where an MHC gene was predicted, the exon boundaries were confirmed by extracting the predicted gene and aligning it to tuatara MHC cDNA sequences SppuU*01 for class I (Miller et al. 2006) or SppuDAB*01 for class II (Miller et al. 2005), then manually editing the exon boundaries as required.
In the course of our work, the first draft chicken genome assembly was released and we aligned it to our GGA2 RH map.
At the end of our work, the first draft chicken genome assembly was released and we aligned it to our GGA5 RH map.
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