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Exact(4)
As mentioned in a previous paper (Duka et al., 2004), INGV provided some personnel and instruments during the performance of measurement campaigns and afterwards with the competence in the modelling procedure.
It is known that Poul Gernes until the mid-1970s had a close collaboration with the Danish paint factory Beck & Jørgensen, and afterwards with the paint factory Flügger and their colour engineer Willum Thomsen (personal information from Poul Gernes' daughter Ulrikka Gernes).
In that case, the membrane was first probed with the lectin and afterwards with the antibody, following the aforementioned protocols.
After rinsing sections were incubated with goat anti-rabbit secondary antibody (1 500; Vector, Burlingame, California, USA) for 30 min and afterwards with the ABC complex/horseradish peroxidase labeled avidin (Dako) also for 30 min.
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Fish were fed daily ad libitum with live Artemia sp. until 12 weeks of age and afterwards with frozen chironomid larvae until the end of the experiment.
These results represented that with 48 h of incubation with NPs, which first leads to cell cycle inhibition and afterwards with increased time of incubation, the nanoparticles supplemented sustained release of drugs, which resulted in apoptosis.
Between trials the set-up was cleaned with 5% ethanol and afterwards with tap water.
Finally, the fibres were extensively washed first in tap water and afterwards with distilled water.
Another strength is that the semistructured interviews helped identify additional questions to include in the survey and, afterwards, helped with the interpretation of the survey results.
The trained reporters took notes during the discussions and afterwards, together with the facilitators, prepared reports on the discussions.
We send educational materials to teachers and then the students come to the play and afterwards, interact with the actors.
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