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Norepinephrine (NE) was the vasopressor of choice and adjusted to a MAP ≥65 mmHg.
Thereafter, the cells were resuspended with 500 μl binding buffer and adjusted to a concentration of 1 × 106 cells/ml.
Glucose and xylose composition, as well as sugar degradation, were monitored and adjusted to a quadratic model.
The inoculum was prepared from 18 h broth culture of each isolate and adjusted to a turbidity equivalent of 0.5 McFarland Standard.
HeLa cells were extensively washed in PBS, counted, and adjusted to a concentration of 107/mL.
Total RNA was quantified and adjusted to a final concentration of 10 ng/µL.
Clods were weighed daily and adjusted to a fixed weight corresponding to the water contents described above.
The extraction was filtered and adjusted to a constant volume (50 ml) with the chloroform:methanol solution.
The cells were washed with PBS, and adjusted to a final concentration of 5×104 cells/10 µl in PBS.
Proteins were dialyzed against a sterile solution of 0.1 M sodium bicarbonate buffer (pH 8.3) and adjusted to a final concentration of 1 mg/ml.
The 10 µm mesh containing the cells was washed out into a 50 mL collection tube with sterile sea water and adjusted to a volume of 40 mL.
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