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Raman spectra were excited by the 633.0 nm line of an HeNe laser in backscattering geometry and acquired with a CCD with 1024 × 1024 pixels.
The current signal, triggered properly, is digitized and acquired with a TDS220 digital oscilloscope (Tektronix, Inc., Beaverton, OR, USA) and transferred to a personal computer through a GPIB where the conductance is calculated and displayed and the cumulative histogram of thousands of consecutive experiments is built.
Data were collected using an Axon 700B amplifier and acquired with a DigiData 1440 (Axon Instruments) at 10 kHz.
Data were digitally sampled at 10 kHz and acquired with a PC using an IOTech acquisition system (Wavebook, IOTech Inc., Cleveland, OH).
Red blood cells were lysed and lymphocytes washed, fixed in 2% p-formaldehyde and acquired with a Cell Quest software by live gating on CD3+ T cells (50000 events) on a four-colour flow cytometer (BD FASCalibur, BD Biosciences, UK).
Images were taken using an inverted microscope Zeiss Axiovert 200 M piloted by Zeiss Axiovision 4.4 software and acquired with a CCD camera Roper Scientific Coolsnap HQ and analysed using the AxioVision LE program.
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CFP and YFP emissions were separated using a Cairn Optosplit II Image splitter and acquired with an Orca-ER digital camera (Hamamatsu, Welwyn Garden City, UK) at 470/24 nm and 535/30 nm, respectively.
It was broken up and acquired, with investors receiving a fraction of what their shares had been worth at the peak.
The next day, coverslips were fixed and stained for immunofluorescence and images acquired with a 63× oil-immersion lens (1.4 NA) on a Leica TCS SP5 confocal system.
All the 1H NMR spectra were recorded with a Bruker Avance 400 MHz spectrometer operating at 300 K and were acquired with a spectral width of 5995.204 Hz, 128 scans and 65536 data-points.
c Reconstructed G-STEM images (forward and backward) acquired with a serpentine scan.
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