In our previous study [6], we showed that a double acceptor consisting of a tandem of Venus and a circular permutation of Venus (cp173Venus; [10]) proved optimal for FRET with mECFP.
Lesions were selected and a circular region of interest (ROI) of 1.5 cm in diameter was placed at the point of maximum uptake, reducing partial volume effects.
The genome has a circular chromosome consisting of 3,940,230 bp with an average G+C content of 30.93% and a circular megaplasmid of 191,996 bp with an average G+C content of 30.91%.
The assembled genome is composed of a circular chromosome of 1,635,045 bp and a circular plasmid of 37,174 bp (supplementary table S2, Supplementary Material online).
The genome of IA3902 contains a circular chromosome of 1,635,045 bp and a circular plasmid of 37,174 bp.
The results are a circular chromosome of 1,288,492 bp and a circular plasmid of 26,406 bp.
These gaps were then closed computationally (by incorporating gap filling reads) or by Sanger sequencing of polymerase chain reaction products derived from gaps yielding a closed circular chromosome of 4.7 Mbp and a circular megaplasmid of 449.8 kb.
Right figure: a circular pattern with serial milling with a through hole of 100 nm (left) and a circular cavity of 60 nm (right), respectively.
At each sampling point, the duff/litter layer was collected when present with a large flat spatula and a circular piece of polyvinyl chloride (PVC) with a diameter of 10 cm.
"Joy eternal" he said with one of his typical old-fashioned rhetorical flourishes, and a circular sweep of the arm.
