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The distribution of intron phase itself showed no direct relation to the ancientness of the sequence since the subset of introns that are located in ancient sequences has a similar phase distribution as introns in the general population (Fig. 7B).
But the Australian study "suggests that if we saw more ancient sequences we might get a very different picture than we get from looking only at the DNA of living populations".
The genetic variability among ancient sequences was quantified as both nucleotide diversity and haplotype diversity.
After their incorporation in our data set (85 sequences) of 122 previously published ancient sequences, the alignment of the 207 ancient sequences produced 78 variable sites (4 of which are indels) defining 113 haplotypes.
In the Iceman system, ancient sequences can be clearly distinguished from contaminants on the basis of nucleotide misincorporation rates.
In contrast, type 2 transitions represent the vast majority (85%) of the observed nucleotide misincorporations in ancient sequences.
Similar(22)
Scattered substitutions, which usually accumulate in aDNA as a result of post-mortem spontaneous damage reactions were observed [36] [41], while consistent substitutions shared by all clones for a given segment were believed to represent the authentic ancient sequence.
On figure 3 (t1 = 0.1), the Fst increases regularly as a function of the proportion of ancient sequence in the second subset (0.001 for n1/nB = 0.1 and 0.085 for n1/nB = 1) with dramatically increasing power (5 % and 72).
The vertebrate tetraspanin family has many features which make it suitable for preserving the imprint of ancient sequence evolution and amenable for phylogenomic analysis.
The vertebrate tetraspanin superfamily has several advantages which make it suitable for preserving the imprint of ancient sequence evolution and amenable for phylogenomic analysis.
The aim of this analysis is to understand how the addition of this single ancient sequence influences the estimates of the timing of the origin of each clade in the genealogy.
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