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In summary, results from the two hybridization experiments anchored a total of 197 markers to the physical map.
Similarly, the second pool hybridization placed 102 markers on the map and anchored a total of 65 contigs.
Among the successful 197 markers used for the hybridization were 87 markers that have been genetically mapped; these markers anchored a total of 54 contigs that cover 76.4 Mb (25.5% of the genome) on all 7 linkage groups (Table 4).
Results from the first pool hybridization anchored a total of 95 drought-induced genes to the physical map with 79 markers hybridized to a single contig, 10 to two contigs, and only 6 markers to three or more contigs (Table 3).
Luo et al. [ 5] generated a genetic map with 7,185 markers (referred to as AL8/78 × AS75 genetic map hereafter) and then anchored a total of 4.03 Gb of D-genome BAC contigs to the map, while Jia et al. [ 21] sequenced Ae. tauschii genome to ~90X depth of short reads, and assembled them into sequence scaffolds which represented 83.4%% of the D-genome.
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Together with the 1,481 (44.8%) physical map contigs that were anchored previously, we were able to anchor a total of 2,880 (87.1%) physical map contigs consisting of 28,416 BAC clones (92.9% of total BAC clones).
Using eight ISSR anchored primers, a total of 38 bands were obtained.
The core of ertapenem, comprising the pyrroline, carboxylate, and hydroxyethyl moieties, is anchored by a total of eight hydrogen bonds to the surrounding protein molecule.
By mapping 120 bp of the flanking sequence surrounding each SNP on to scaffolds of ATLCOD1A build of the cod genome (Star et al. 2011) using BLASTN with an e-value threshold of 1−10, 133 additional SNPs could be anchored (for a total of 899 of the SNPs) on to specific linkage groups (LGs), although the position within LGs (in cM) could not be determined for these SNPs.
On each vertex, the midpoint of each side, and the center of the square, there is an anchor (a total of 9).
In addition, 21 other sequence characterized mapped markers were homologs of single copy genes in Arabidopsis and thus were effective anchors, making a total of 102 mapped anchor markers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com