Sentence examples for anchor sequences from inspiring English sources

Then we applied amplification based on anchor sequences at both ends, with higher annealing temperature, to boost the yield of the final product.

In TTAS method, PCR anchor sequences are introduced by terminal elongation instead of ligation, which has been known to suffer from unsatisfied reaction efficiency (Cao et al., 2017).

Ligation of anchor sequences was done according to the instructions of the manufacturer.

The signal anchor sequences of rat sialyl transferase (ST) were PCR amplified from plasmid ST-GFP [42].

The two former have anchor sequences, while the latter is strongly predicted to harbor a signal peptide.

Genomic DNA libraries of S. mansoni were made and screened by a PCR approach using nested-primers against defined anchor sequences and the known diaphanous 5'-cDNA end.

An unexpected finding was that PspC variants could differ in the anchor sequence.

The second strand was synthesized using a different oligo-dT primer with a different anchor sequence, and the cDNA was amplified by PCR.

VSPs are characterized by an extremely conserved C-terminal membrane spanning sequence and short hydrophilic cytoplasmic anchor sequence, CRGKA.

Target selection by the gRNA is governed by the anchor sequence (4 16 nts) at the 5' end of the molecule.

The resulting AfOch1* is predicted to contain a non-cleavable N-terminal anchor sequence (Signal peptide probability: 0.117; Signal anchor probability: 0.883).