To prevent cleavage of the anchoring segment and increase the long-term stability of zwitterionic polymer-based brushes in seawater, Vancso and co-workers investigate diblock copolymer-type brushes composed of bottom hydrophobic segments and a polysulfobetaine top on the substrate of SiOx (Fig. 9) [74].
Similarly, the NPC-anchor segment of Nup153 remained attached to the NE, as did residual copies of Nup98's NPC-anchor domain.
A ∼1.1 kbp anchoring segment was amplified, by PCR, from plasmid pRL574 using a digoxigenin-labeled primer and then subsequently digested with BstXI (NEB, Ipswich, MA) to produce an overhang.
After the ligation of the anchoring segment and unzipping segment containing a nucleosome, we incubated 20 nM of the nucleosomal DNA with 200 nM Gal4DBD at 16°C for 30 min. Single molecule sample preparation was performed according to protocols previously described (Li and Wang, 2012).
ES is bound to the membrane of the endoplasmic reticulum, presumably through multiple transmembrane and other membrane anchoring segments.
Mr. Smith defended the anchors' segments, known as "must-runs," and likened them to the late-night shows that networks air on their local affiliates.
Recent experimental, bioinformatics and molecular modelling data showing the primary, secondary and quaternary organization of the HA and F anchoring segments are summarized in this review.
In contrast, their anchoring segments, which possess a linker region, transmembrane domain and cytoplasmic tail that is specifically modified with long fatty acids (highly conserved in HA and occasional in F), are not resolved.
