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For grasses, the database contains 1335 candidate anchor loci.
Overall, the total number of anchor loci amounts to 77 markers.
Such markers were considered as anchor loci, around which the map was developed.
The segregations of SSR anchor loci were determined in an earlier study of the same mapping population [ 7].
Linkage groups were assembled into chromosomes based on the presence of anchor loci - SSR (Table 2) and DArT.
Six maps from core recombinant inbred line (RIL) mapping populations were chosen for building a reference consensus maps using common (anchor) loci.
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This is efficiently done because Socrates keeps track of the anchor locus consensus sequences.
The aim was to enrich linkage groups to achieve a joining (anchor) locus density <50 cM per joining locus.
Since we found evidence of recombination within the Hox1 anchor locus, we attempted to further extend these investigations.
The following primers were used to amplify the Hox1 anchor locus: forward, 5′-CATTCATTCATTCATTCGCCCTC-3′ and reverse, 5′-CGGTCTCGCCAGTTGTATCAAG-3′ (Tm = 58 °C).
Moreover, some fragments did not match the sizes expected for the anchor locus but were found to be significantly larger (fig. 2 A ).
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