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The definition of 'Core' HG (Supplementary Note 1, Supplementary Data 2) allows for absence of the HG in one member of the ingroup to accommodate incompleteness of some genomes; ancestral lists of HG are therefore not affected by these genomes, as these HG are defined by their presence in at least two genomes belonging to the two main lineages within a clade.

A similar trend was seen using the proportion of ancestry-associated SNPs (Cochran-Armitage model, Table S2) suggesting that relative to the geo-ancestral list, other factors not defined by these eQTLs may contribute to the differential expression of metabolic genes.

We construct an ancestral COG list using the probability 0.7; whenever the probability level is not stated, we refer to LUCA-ML 0.7 as LUCA-ML.

However, out of this metabolic cluster only PYGL had a proportion of eQTL (number of eQTL per total number of gene SNPs, 3.8%, additive model p<0.01) greater than the mean proportion of eQTL from the entire geo-ancestral gene list (3.3%).

In order to detect apparent homoplasies when the species tree is assumed, we used CODEML to reconstruct ancestral sequences and list nucleotide changes occurring along each branch (with settings model = 1 to allow each branch its own dN/dS value, and NSsites = 0 to allow only one category of site).

There are 21 accessions belong to 80 ancestral soybean lines listed by Gizlice et al. [ 5].

For all SNPs where it can be inferred, the ancestral state is listed in Table S3.

The "human-specific" families of TEs are defined as those present in humans and in primates ancestral to humans (listed in Additional file 1: Table S1, col. A).

The first known attempt at a taxonomy for EPAs was originally made by Mills (2003), who proposed a classification system for EPAs that included a "list of ancestral problems and subsequent putative mechanisms".

This study, in combination with the independent characterization of post-mating expression profiles in females and the relationship between transcript abundance and female fitness in the ancestral population, provides a list of genes associated with changes in female fecundity caused by evolution under different sexual selective pressures.

Unlike all other secondary and tertiary endosymbionts, however, the dinotom endosymbiont maintains a long list of ancestral characters, including a large nucleus and many mitochondria (Tomas et al. 1973; Jeffrey and Vesk 1976; Horiguchi and Pienaar 1991, 1994; Tamura et al. 2005; Pienaar et al. 2007; Takano et al. 2008).

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