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To verify this result, we used an alternative marker of autophagy by analyzing the expression of Atg5-containing protein complexes during development.
The onset of osteoblast formation was evaluated by analyzing the expression of alkaline phosphatase (ALP).
When analyzing the expression of axin2 and sprouty2, the same results were observed (fig. 1K P).
Thus, analyzing the expression of proinflammatory mediators may facilitate identification of anti-inflammatory substances.
We further investigated the apoptotic response to doxorubicin by analyzing the expression of other proapoptotic proteins.
We further analyzed the expressions of the 65 new miRNAs under the two treatments.
RT-PCR was used to analyze the expressions of several genes of interest.
For this reason we analyzed the expression of MyoD and Myf5 in mDUX-expressing myoblasts.
Western blotting analyzed the expression of Kif4A and CCR2.
Thus, we analyzed the expression of tendon-associated genes in the tenocytes.
We also analyzed the expression of deiodinase-3 (dio3), an enzyme implicated in progenitor cell proliferation22,23.
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