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A procedure for graphical chain modeling has been designed for analyzing the expression profiles of genes that can be classified into several blocks in a natural order.
The myogenic differentiation potential of dt versus wild-type cells was monitored by examining the expression of myosin heavy chain by immunofluorescence, and by analyzing the expression profiles of myogenic regulatory factors and myogenic differentiation markers by RT-PCR.
An illustrative example is presented by analyzing the expression profiles of the gene family involved in sulfur- and sulfur containing amino acid-metabolism in yeast.
We studied hnRNP K-regulated MMPs by analyzing the expression profiles of MMP family genes in NPC tissues and hnRNP K-knockdown NPC cells using Affymetrix microarray analysis and quantitative RT-PCR.
Long et al. [ 67], analyzing the expression profiles of 70 archived FFPE tumor specimens, showed that miR-519d and miR-647 could serve as biomarkers to discriminate between patients with and without BCR following RP.
Therefore, exploring and analyzing the expression profiles of pTyr proteins in HCC cells by a high-throughput method would be extremely useful for obtaining insights into the mechanisms of HCC metastasis and recurrence.
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Analyzing the expression profile day 4 6 separately, however, provided us with 760 probes differentially expressed with the majority of genes induced by PPARγ (Spreadsheet S2), 222 probes of which were not present in the set including all time-points.
Analyzing the expression profile, we found miR-144 down-regulated in cancer, but up-regulated in Parkinson disease and idiopathic Myelofibrosis.
Microarray data were validated by analyzing the expression profile at 0, 2 and 24 h excess light stress.
The signal values of those expressed COEs were clustered to analyze the expression profiles.
To analyze the expression profiles of angiogenesis-related proteins, we used the Proteome Profiler™ Human Antibody Array Kit (R&D Systems Ltd ,Abingdon, UK), according to the manufacturer's instructions.
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